BACKGROUND OF DIURETIC ACTIVITY OF DRUGS
The aim of the study was to screen the diuretic activity of given test drug.
Diuretics are the drugs that increase urine output and help in treating mild to moderate hypertension by reducing volume of the plasma and thus venous return to the heart.1
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REQUIREMENTS
Animals: Male Albino Wistar rats of 140-200 g of either sex.
Drugs: 2% CMC in normal saline- 10 ml/kg
Furosemide- 10 mg/kg, p.o in vehicle.
Instruments: PH meter
Volumetric tubes.
PROCEDURE
Divide selected male Albino Wistar rats into two groups consisting of 3 animals in each. Group-1 receives 2% CMC in normal saline and serves as control and the other group receives Furosemide and serves as test group. After treatment immediately hydrate the rats with 15 ml/kg of saline and place them in metabolic cages separately and maintain the room temperature to 21±0.5oc and avoid access to feed and water. Care to be taken to avoid mixing of urine and faeces. After 5 hours measure the total volume of urine collected in measuring cylinder and compare the same in two groups. Other parameters like urine pH and concentration of electrolytes such as sodium, chloride and potassium are to be estimated by using ion selective electrode method described by the manuals in biochemical kits.2
OBSERVATION
| Group | Parameters | ||||
| Urine volume (in ml) | Urine pH | Concentration of sodium (mmol/L) | Concentration of potassium (mmol/L) | Concentration of chloride (mmol/L) | |
| Group-1 (2% CMC in normal saline) | |||||
| Group-2 (Furosemide- 10 mg/kg, p.o.) | |||||
CONCLUSION
Furosemide is a loop diuretic and acts by inhibiting sodium-potassium-chloride co-transporter. It increases urine output with increase in concentration sodium, potassium and chloride in urine. It plays an important role in relieving hypertension.
REFERENCES
- Jayasree T, Kiran KK. Evaluation of the diuretic effect of the chloroform extract of the Benincasa hispida rind (pericarp) extract in guinea pigs. J Clini Diagnos Res. 2011;5(3):578–82.
- Sayana SB, Khanwelkar CC, Nimmagadda VR, Dasi JM, Chavan VR, Kutani A, et al. Evaluation of diuretic activity of alcoholic extract of roots of cissampelos pareira in albino rats. J Clin Diagn Res. 2014;8(5):1-4.
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