Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.
Adherent cells needs to be subculture after getting confluency (more than 70%) in the plates without it cells will remain unhealthy and sensitive for contaminations. We concluded the trypsinization and cell counting of the cells with the help of haemocytometer.
Isolation of total RNA from the animal cells is an essential step to initiate any experiment like cDNA synthesis, real-time PCR, RNA sequencing etc. When we isolate RNA is comes with all kind of RNAs from the animal cells. Trizol kit is very famous to apply for RNA isolation easy.
Assay fragmented DNA is essential to investigate the apoptosis. TUNEL assay is a method to detect fragmented DNA in apoptotic cells grown on the coverslip.
Transient transfection method introduces foreign nucleic acids (DNA) into cells to produce genetically altered cells for a defined period of time and an essential tool for introducing a gene function
To estimate the toxicity of drugs and chemicals, cell viability assay is an appropriate method. Assay for quantitative cytotoxicity can be performed in various methods to determine the cell viability- MTT assay, LDH assay, neutral red assay, and calcein assay
Western blotting is an essential technique to analyze protein in various studies such as cellular and molecular biology and biochemistry in biomedical research.
To maintain the cell culture stock, we store integrated viable cells in cryovials in liquid N2 tank for the application in future. It saves all expensive chemical resources for the cell culture also. In this experiment, we aimed to cryopreserve healthy and integrated cells in liquid N2 for future usage.
Cryopreserved animal cells in the liquid N2 can be thawed anytime for further applications. Thawing and harvesting cells is important for reviving cells after long time storage. The principle aim of this method is thawing healthy, cryopreserved, and integrated cells for future application.
Culturing cells and its maintenance is as important as cell growth control. Cells grow fast and there will be so many plates after many passages. We have to preserve cells for further use in future, for that,
Gelatin, a mixture of heterogeneous, water-soluble proteins present in collagen and isolated by boiling the tendons, ligaments, bones, and relevant skin with water.
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