Quiz 29-January-2024

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Labmonk quiz, NET Life Science,

#1. The pH of blood of a healthy person is maintained at 7.40 ± 0.05. Assuming that this pH is maintained entirely by the bicarbonate buffer (pKa1 and pKa2 of carbonic acid are 6.1 and 10.3, respectively), the molar ratio of [bicarbonate]/[carbonic acid] in the blood is

#2. Most common type of phospholipid in the cell membrane of nerve cells is

#3. In contrast with plant cells, the most distinctive feature of cell division in animal cells is

#4. Michaelis and Menten derived their equation using which of the following assumption?

#5. Equilibrium constant (K) of noncovalent interaction between two non-bonded atoms of two different groups was measured at 27°C. It was observed that K = 100 M–1. The strength of this noncovalent interaction in terms of Gibbs free energy change is:

#6. Maturation-promoting factor (MPF) controls the initiation of mitosis in eukaryotic cells. MPF kinase activity requires cyclin B. Cyclin B is required for chromosome condensation and breakdown of the nuclear envelope into vesicles. Cyclin B degradation is followed by chromosome de-condensation, nuclear envelope reformation and exit from mitosis. This requires ubiquitination of a cyclin destruction box motif in cyclin B. RNase-treated Xenopus egg extracts and sperm chromatin were mixed. MPF activity increased with chromosome condensation and nuclear envelope breakdown. However, this was not followed by chromosome de-condensation and nuclear envelope reformation because

#7. The erythrocyte membrane cytoskeleton consists of a meshwork of proteins underlying the membrane. The principal component spectrin has α, β-subunits which assemble to form tetramers. The cytoskeleton is anchored to the membrane through linkages with the transmembrane proteins band 3 and glycophorin C. The cytosolic domain of band 3 also serves as the binding site of glycolytic enzymes such as glyceraldehyde 3-phosphate dehydrogenase. Analysis of the blood sample of a patient with haemolytic anaemia shows spherical red blood cells. The patient carries

#8. The hydrolysis of pyrophosphate to orthophosphate is important for several biosynthetic reactions. In E. coli, the molecular mass of the enzyme pyrophosphatase is 120 kDa, and it consists of six identical subunits. The enzyme activity is defined as the amount of enzyme that hydrolyzes 10 μmol of pyrophosphate in 15 minutes at 37°C under standard assay condition. The purified enzyme has a Vmax of 2800 units per milligram of the enzyme. How many moles of the substrate are hydrolysed per second per milligram of the enzyme when the substrate concentration is much greater than Km?

#9. The membrane lipid molecules assemble spontaneously into bilayers when placed in water and form a closed spherical structure known as

#10. On the molar scale which of the following interactions in a nonpolar environment provides highest contribution to the biomolecule?

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