Determination of TDP & TDT of E.coli for designing of a sterilizer
The study concludes that the thermal death point and thermal death temperature of E. coli for sterilizer design.
Microbiology
Screening for citric acid producing organisms
All aerobic organisms naturally contain the organic acid citric acid (2-hydroxy-1, 2, 3-tricarboxylic acid), which is formed during cellular respiration.
Effect of inhibitors on enzyme activity
Enzyme inhibitors are substances that change how an enzyme catalyses, slowing down or, in some cases, stopping catalysis as a result
Methods for purification of enzymes
Enzymes are substances that operate as catalysts in living things, controlling the pace at which chemical
Isolation of O & H Antigen from Salmonella typhi
Aim: Isolation of O & H Antigen from Salmonella typhi BACKGROUND Typhoid fever, paratyphoid fever, and other food-borne illnesses are all brought on by the Salmonella genus of rod-shaped, Gram-negative, non-lactose fermenting Enterobacteriaceae. Subspecies enterica serotypes Paratyphi A, B, and C are responsible for paratyphoid fever. The Widal test is the most popular diagnostic technique … Read more
Microbial Staining Methods
Aim: Microbial Staining Methods BACKGROUND The most of bacteria have refractive indices that are similar to those of the aqueous fluids in which they are floating and are mostly transparent and colourless. For visualization of the bacteria, we use stain and dyes. To improve the clarity of the microscopic image, staining is an adjunct technique … Read more
Affinity Purification of Immunoglobulin
Aim: Affinity Purification of Immunoglobulin BACKGROUND Numerous in vivo and in vitro applications of antibodies include drug targeting, the development of immunodiagnostics, immunoprophylactic, and immunotherapeutic, the detection of foodborne pathogens, adulterants, toxins, and residues in food samples, environmental analysis/monitoring, and the isolation of a variety of proteins and other targets for basic research and industrial … Read more
Preparation of enzyme conjugated antibodies
Antibody conjugation protocol, enzyme conjugated antibody, antibody conjugation methods, enzyme-conjugated antibody elisa, conjugated enzymes, function of enzyme conjugate in elisa, antibody production steps, mechanism of antibody production pdf Aim: Preparation of enzyme conjugated antibodies BACKGROUND The enzyme-antibody conjugation is important tool in immunochemistry. Although certain enzymatic conjugations, like sortase-based protein terminal conjugation or even through … Read more
Purification of Immunoglobulin by Precipitation
BACKGROUND Glycoproteins termed immunoglobulin make up antibodies. A protein called immunoglobulin that binds to antigens is found on the B-cell membrane and is released into the serum by plasma cells. B-lymphocytes create immunoglobulins in response to external antigens. Using the protease enzyme, Rod Porter and Gerry Edelman described the immunoglobulin structure (Papain and Pepsin). Two … Read more
Microplate based Alamar Blue Assays
Microplate based Alamar Blue Assay (MABA) is a practical, sensitive and inexpensive assay method of cell viability in which fluorescence reduction assay of alamar blue
