Biochemical Test (IMViC reactions)
IMViC test is performed for identification of bacteria after doing gram staining. These are one of the stable reactions which gives more accurate results than other tests.
Microbiology
Study of bacterial morphology by acid-fast staining (Ziehl and Neelsen staining method)
BACKGROUND Acid-fast staining is one of the widely used differential …
Mutagenesis with Ethidium bromide/NTG or EMS
BACKGROUND Mutagenesis is a valuable technique in modern biology and …
Preparation of Standard Solution
In every analytical laboratory, it is highly essential to keep stocks of solutions of various reagents, among which some are of accurate concentration which we call standard solutions.
Isolation of antibiotic resistant Spontaneous mutant
BACKGROUND A heritable alteration to the DNA’s nucleotide sequence is …
Examination of Urine by Culture and Isolation of Human Pathogen (Bacteria) & Determination of Antibiotic Sensitivity Pattern of the Isolated Bacterial Strain
Urine culture is a type of laboratory test which is utilized to estimate presence of bacteria and germs in the urine sample. It is done based on the complete urinalysis test.
Isolation and Enumeration of Bacteriophages (PFU) from Water Sewage Sample Using Double Agar Layer technique
The basic objective of this test is to study about the isolation and enumeration of bacteriophages from water sewage samples by using a double layer agar technique.
Estimation of RNA Using UV Spectrophotometer (A260 measurement)
The concentration of RNA present is directly proportional to the absorbance of light carried out in a spectrophotometer. This Beer Lambert’s Law.
Estimation of DNA and its purity check using UV spectrophotometer (A260 measurement)
Estimation of DNA can be done by using various method like absorbance, agarose gel electrophoresis etc. Among all estimation using spectrophotometer is convenient
Determination of Thermal Death Point of bacteria
Thermal death point (TDP) of bacteria is basically the study of effect of heat on the growth of bacteria. In simple terms it is the time needed to kill bacteria in a medium
