Synthesis of 1,3-substituted pyrazole (i.e. 1,3-diphenyl pyrazole) from diarylhydrazone (i.e. diphenyl hydrazone) and vicinal diol
1,3-substituted pyrazole is prepared by cyclization of diarylhydrazone and vicinal diol in presence of ferric chloride and tert-butylhydroperoxide(TBHP) which is also called regioselective synthesis of substituted pyrazole
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry and molecular biology to separate proteins according to their electrophoretic mobility
The principle behind this experiment is the reaction between diphenylamine and deoxyribose sugar that produces blue colour complex.
Chromatography is one of the most important techniques for separating closely related compounds present in a mixture. The separation becomes effective
Of any biological system enzymes are the catalyst and they are very efficient as well as specific like that of catalysts. Then to it add about 1 ml of the amylase solution.
The principle involved in Lowry method is determining the protein concentration by calculating the reactivity of the peptide nitrogen with the Copper ions under alkaline conditions
RNA content of any plant tissue can be estimated by this procedure. RNA abbreviation is ribonucleic acid is a complex compound of high molecular weight that functions
Isolation of genomic DNA is one of the most important and common experiment that is carried out in molecular biology and includes the transition from cell biology to molecular biology.
Estimation of DNA can be done by using various method like absorbance, agarose gel electrophoresis etc. Among all estimation using spectrophotometer is convenient
The concentration of RNA present is directly proportional to the absorbance of light carried out in a spectrophotometer. This Beer Lambert's Law.