Quantification of Danazol by Ultraviolet Spectrophotometer in Capsules

BACKGROUND OF DANAZOL

Danazol, chemically is a synthetic steroid used to treat primarily endometriosis. Besides this, Danazol is also used for symptoms like immune thrombocytopenic purpura, hereditary angioedema. In present procedure simple Ultraviolet method applied to determine the quantity of Danazol in capules.¹ 

The molecular formula and molar mass_.is C₂₂H₂₇NO₂ and 337.5 g/mol respectively. Due to its significant side effects the drug is generally not prescribed for more than six months.

REQUIREMENTS

• Danazol API
• Danazol capsules
• 100 ml volumetric flasks
• Chloroform
• 0.45 µm filter paper
• Ultraviolet Spectrophotometer
• Glass Bealker/ Suitable glass container

PROCEDURE

Standard solution

Weigh accurately 10 mg of Danazol API in 100 ml volumetric flask containing about 60 ml of chloroform, mix well by gentle shaking and make up the volume up to 100ml by chloroform.

Sample solution

Open and mix the contents of 20 no of Danazol capsules (market brand) in suitable glass container. Weigh and add capsule content powder equivalent (considering lable claim) to 10 mg of Danazol in 100 ml volumetric flask containing about 50 ml of chloroform, shake vigorously and make up the volume up to the mark by chloroform. Filter sample solution by 0.45 µm filter paper.

Instrumentation

Before starting readings

Stable the baseline without standard or sample solution in cuvette with cover closed. Auto zero the instrument by taking chloroform in both reference and sample cuvettes. Take standard solution in cuvette (1^st slot) and take three readings at 285 nm by taking standard solution each time from volumetric flask. Clean the cuvette by chloroform. Take three reading for filtered sample solution at 285 nm by taking sample solution each time from volumetric flask.

Sl. No.	Standard Absorbance	Sample Absorbance
1
2
3
Avg.

Calculation

% Assay= Avg. sample absorbance/ Avg. Standard absorbance x 100

CONCLUSION

The assay of danazol in capsules (market brand) tablets found to be ____ %.

REFERENCES

1. Indian Pharmacopoeia.

Also read:

• Calibration of pH meter
• Assay of Azithromycin by HPLC in tablet dosage form
• Assay of Cefadroxil by HPLC in tablet dosage form
• Preparation and standardization of sulfuric acid
• Determination of the concentration of potassium ion using Flame Photometry
• Preparation and standardization of ceric ammonium sulphate
• Acute toxicity testing for topical preparations
• Calculation of Body Surface Area (Using a Nomogram)
• Estimation of white blood cell count (TLC/ Total Leucocyte Count)

🔴 Would you like to attempt Labmonk Daily quiz? Click here

🔵 Check out Jobs & Exam Notices. Labmonk Notice Board

🔴 Labmonk Scholarships. Click here

🔵 Labmonk Blog. Click here

🔴 Do you need notes? Click here