BACKGROUND
Azithromycin is administered in different dosage forms to as antibiotic for the treatment bacterial infections. Few of the symptoms includes intestinal infections, middle ear infections, throat infection, pneumonia, traveler’s diarrhea, and overall for respiratory track infections.1 The Molecular weight and formula are 748.996 g/mol and C38H72N2O12.
Aim of the experiment is to determine the content of Azithromycin in tablet dosage form is elaborated which is simple by using HPLC with ultraviolet detector.2
REQUIREMENTS
- Azithromycin Active Pharmaceutical Ingredient
- Azithromycin tablets
- Dipotassium hydrogen phosphate
- Phosphoric acid
- Acetonitrile
- Volumetric flask
- Mortar pestle
- Weighing balance
- 0.45 µm filter paper
- Water
- High Performance Liquid Chromatography
- Column [C18 column (15 mc x 4.6 mm, 5 μm)]
PROCEDURE
Buffer solution
Dissolve 1.16 gm of dipotassium hydrogen phosphate in water and dilute to 100 ml with water. Adjust pH to 6.5 with phosphoric acid.
Mobile phase
Mix buffer : acetonitrile : water in the ratio of 10:35:55
Diluent
Mix acetonitrile : water in the ratio of 40:60
Standard solution
Weight 20 mg of Azithromycin (API) in 100 ml volumetric flask containing about 50 ml of diluent, mix well and make up the volume up to 100 ml by diluent.
Sample solution
Triturate 20 no of azithromycin tablets in a mortar pestle. Weight equivalent to 20 mg of powdered sample in a 100 ml volumetric flask containing about 50 ml of diluent, shake vigorously to dissolve complete for about 10 -15 minutes, make up the volume up to 100 ml by diluent. Filter sample solution by 0.45 µm filter paper.
Chromatographic conditions
C18 column (15 mc x 4.6 mm, 5 μm)
Column temperature 70°C
Mobile phase flow rate 1 ml/min
Detection wavelength 215 nm
Injection volume 100 μl
Run the HPLC only with mobile phase at least for 10 minutes for conditioning of column.
Now inject standard solution six times by taking solution from volumetric flask each time and record the response/area.
Similarly inject filtered sample solution six times by taking solution from volumetric flask each time and record the response/area.
| Sl. No. | Standard Area | Sample Area |
| 1 | ||
| 2 | ||
| 3 | ||
| 4 | ||
| 5 | ||
| 6 | ||
| Avg. |
Calculation
Content (mg/tab)= 
CONCLUSION
The content (mg/tab) of Azithromycin (market brand) tablet found to be ____.
REFERENCES
- https://medlineplus.gov/druginfo/meds/a697037.html
- Indian Pharmacopoeia.
Also read:
- Preparation and standardization of sulfuric acid
- Determination of the concentration of potassium ion using Flame Photometry
- Preparation and standardization of ceric ammonium sulphate
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- Calculation of Body Surface Area (Using a Nomogram)
- Estimation of white blood cell count (TLC/ Total Leucocyte Count)
- Determination of the concentration of potassium ion using Flame Photometry
- Mutagenesis with Ethidium bromide/NTG or EMS
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