BACKGROUND
Insulin
Insulin is a peptide hormone produced by the beta cells of pancreas in response to high glucose levels in the blood. Released insulin acts on the insulin receptors on body cells and activates glucose transporters to absorb more glucose into the cells thereby regulates carbohydrate, protein and fat metabolism in body cells.
Reduced blood glucose levels inhibit insulin release and stimulate alpha cells of pancreas to release glucagon to maintain glucose levels in the blood by glycogenolysis and gluconeogenesis.1 The aim of the present study is to evaluate the effect of insulin in rabbits at different time intervals.
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REQUIREMENTS
Animals required: Healthy rabbits weighing 1800-3000 gms.
Drugs: 20 units of insulin preparation. One unit contains 0.04082 mg of insulin
Reagents: Normal saline, HCl, 0.5% phenol, 1.4-1.8% glycerin.
PROCEDURE
Select healthy rabbits weighing 1800-3000 gms for the study. They should be maintained in uniform diet for 7 days. Fast the animals for 18 hrs with no access to water before starting the procedure. Select three animals for the study and inject 1 unit/ml of insulin.2
Prepare drug solution freshly. Weigh 20 units of insulin accurately and dissolve it in normal saline. Acidify the solution by using HCl to pH 2.5. Add 0.5% of phenol as preservative and 1.4-1.8% of glycerin and make the final volume to 20 units/ml of solution.
Withdraw 2 ml of blood from marginal ear vein of each rabbit and estimate blood glucose level by using suitable biochemical method and the concentration of glucose can be noted down as initial blood glucose level.
Then inject insulin (1 unit/ml) to the animals and check the blood sugar level up to 5 hours at the interval of 1 hour each and the determine blood glucose levels as final blood sugar level and compared both initial and final blood glucose levels.
OBSERVATION
| Animals | Initial blood glucose level (in mg/ml) | Final blood glucose level at different time intervals (in mg/ml) in hours | ||||
| 1 | 2 | 3 | 4 | 5 | ||
| 1st animal | ||||||
| 2nd animal | ||||||
| 3rd animal | ||||||
| Average mean value | ||||||
CONCLUSION
Mean percentage decrease of blood glucose levels at different time intervals determines the effect of insulin.
REFERENCES
- Lubert S. Biochemistry. Fourth edition. New York: W.H. Freeman and Company; 1995: 773–774.
- Goyal RK. Principles and Methods of Bioassay. Ahmedabad. 2008: 14-16.
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