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BACKGROUND
The pour plate method is a common method for isolation of pure cultures and also for counting the number of microbial colonies. So, the objective of this method is to study how to isolate pure cultures by pour plate method.
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REQUIREMENTS
Materials: Test microbial sample
Sterile water
Equipments: Sterile capped test tubes
Agar medium
Flame
Pipettes
Petri plates
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PROCEDURE
First make the dilution of the test microbial sample. Accordingly, mark the plates in which dilution you have to pour.1 Then inoculate about 0.1 ml of the diluent in the molten agar and mix it thoroughly. Now take the sterile molten agar and remove the cap of the container or the cotton plug in left hand. Now show the neck of the flask in the flame and lift the lid of the petri plate and then pour the molten agar in the plate uniformly and cover the lid. Slowly rotate the plate so that the agar is distributed in the plate in a very evenly manner. In most of the times the agar migrates towards one side of the plate. Try to avoid this by rotating the plate.Then allows the plates to solidify. As soon as the plate settles down incubate the plate at the required temperature and duration.
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CONCLUSION
Although it has few limitations for isolation of pure cultures but it is a very simple method to follow.
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REFERENCES
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Basic Practical Microbiology- A manual by society for General Microbiology.
