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BACKGROUND
In microbiology one of the fast-qualitative methods for the isolation of pure culture is streak plate method. Although there is a number of streaking method but the most common method is quadrant streak method. So, the basic objective is to isolate the pure microbial cultures from inoculum.
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REQUIREMENTS
Materials: Microorganism source like stock culture, agar plate
Pure agar plate.
Equipments: Inoculating loop
Bunsen burner
Lysol
Paper towels
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PROCEDURE
First sterilize the inoculating loop by keeping the tip of loop in the flame of Bunsen burner until it is red hot. Let it cool down. Then pick a microbial colony from the stock culture and then streak the first quadrant as four parallel streaks. Show the loop to flame and then let it cool down.Then go to the edge of each line of the first quadrant and extend this to second quadrants sets.1 Again, sterilize the loop and in the same manner streak the third set of quadrants. Repeat the technique for the fourth set of quadrants. Show the inoculating loop to the flame and keep it. Now close the inoculated plate carefully to avoid contamination. Then incubate the plates for growth. After growth the plate should be checked for the purity of culture by seeing that pure isolated colonies are formed towards the last quadrant. These pure isolated colonies can be again subcultured and kept in slants or they can be used for studying various properties.
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CONCLUSION
Streak plate method is one of the most common methods for the isolation of pure cultures of all microorganisms.
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REFERENCES
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VanSoestbergen AA, Lee CH. Applied Microbiology, 1969. Available at: ncbi.nlm.nih.gov.
