Great attention has been given recently to some relatively rapid techniques of analysis. Thin layer chromatography (T.L.C.) is already accepted and established as a laboratory tool for routine work. Its low cost, ease, and rapidity along with its capacity for separating and identifying small
Chromatography is used to separate mixtures of substances into their individual components. All forms of chromatography work on the same principle. They all have basic requirements of stationary phase (a solid or a liquid supported on a solid)
Wet pack method is used to prepare the column. The chromatography column is made with plastic tip with frit, the one-way stopcock, and the plastic funnel. The column is filled with enough alumina to get the required height.
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