Labmonk quiz, NET Life Science,
#1. A culture of an E. coli strain that is lysogenic for phage lambda is grown at 32°C. Induction of the prophage from the host chromosome will occur when the culture is exposed to
#2. The fidelity of replicative base selection can be reduced by a factor of 102 when the repair of DNA synthesis involves
#3. Budding yeast cells that are deficient for Mad2, a component of the spindle-attachment-check point, are killed by treatment with benomyl, which causes microtubules to depolymerise. In the absence of benomyl, however, the cells are perfectly viable. Which explanation out of the following can justify this observation?
#4.
Lac repressor inhibits expression of genes in lac-operon whereas purine biosynthesis is repressed by the Pur repressor. The two proteins have 31% identical sequences and have similar three-dimensional structures. The gene regulatory properties of these proteins differ in relation to-
P. binding of small molecules to the repressor.
Q. presence of recognition sites on the genome.
R. oligomeric nature of the repressor.
S. DNA binding property.
The correct statements are:-
#5. Both halophytes and glycophytes compartmentalize cytotoxic ions into the intracellular compartment or actively pump them out of the cell to the apoplasts with the help of membrane transport proteins. Among these, the Na+- H+ antiporter, NHX1, is localized in the
#6. The 5′ cap of RNA is required for the
#7. What is the minimum number of NTPs required for the formation of one peptide bond during protein synthesis?
#8.
In eukaryotic chromatin, 30 nm fibre (solenoid) can open to give rise to two kinds of chromatin. In one type (A), the promoter of a gene within the open chromatin is occupied by a nucleosome whereas in the other (B), the promoter is occupied by histone H1. The following possibilities are suggested.
P. The gene in (A) is repressed
Q. The gene in (B) is repressed
R. The gene in (A) is active
S. The gene in (B) is active
Which of the following sets is correct?
#9. Eukaryotic genomes are organized into chromosomes and can be visualized at mitosis by staining with specific dyes. Heat denaturation followed by staining with Giemsa produced alternate dark and light bands. The dark bands obtained by this process are mainly
#10. During receptor-mediated endocytosis, apolipo-protein B on the surface of an LDL particle binds to the LDL-receptor present in coated pits containing clathrin. The receptor-LDL complex is internalized by endocytosis, trafficked to lysosomes and the LDL-receptor is finally recycled. A patient report with familial hypercholesterolemia. This could be due to
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