Pharmaceutical Analysis

Ascending Paper Chromatography

Chromatography is used to separate mixtures of substances into their individual components. All forms of chromatography work on the same principle. They all have basic requirements of stationary phase (a solid or a liquid supported on a solid)

Separation of plant pigments by column chromatography

Wet pack method is used to prepare the column. The chromatography column is made with plastic tip with frit, the one-way stopcock, and the plastic funnel. The column is filled with enough alumina to get the required height.