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BACKGROUND
Isolation and identification of bacteria is a very important process in every microbiological lab. It involves various techniques that helps in isolating two different unknown bacteria. The importance of identification of bacteria is not only confined to microbiological labs but also in various other sectors like medical, industrial etc.1 Therefore, the basic objective of this test is to isolate and identify two unknown bacteria by morphological and biochemical methods.
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REQUIREMENTS
Organism: Bacterial culture
Instruments: Inoculation loop
Bunsen burner
Incubator
Media: Nutrient agar plate
Nutrient broth
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PROCEDURE
In a sterilized inoculation loop take a loopful of each bacterial culture and inoculate in nutrient broth present in a test tube. Incubate it at 370C for about 24 hours.1 After 24 hours, observe if growth has occurred or not in the broth, if not then keep it for another 24 hours. If growth has occurred then by using aseptic conditions, take loopful culture and streak in the nutrient agar plate. Then isolated colonies are taken and streaked in nutrient agar slants for further studies like IMViC test, Nitrate reduction test, catalase test, oxidase test. The morphology of two bacteria is studied using microscope.
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CONCLUSION
Depending on the results of the above test, the bacteria can be easily identified. While morphological test gives idea about the shape and structure of the bacteria, in the other hand biochemical test gives idea about different properties of bacteria.
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REFERENCES
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Muyzer G, Teske A, Wirsen CO, Jannasch HW. Achieves Of Microbiology, Springer. 1995.
