Quiz 27-October-2022

#1. Which of the following technique is of no use in protein engineering?

X-ray crystallography

Lyophilization

Protein sequencing

Gene cloning

#2. Which of the following is the first and the most important step in the polymerase chain reaction?

None of the above

Annealing

Primer extension

Denaturation

#3. The PCR technique was developed by

Milstein

Altman

Altman Kary Mullis

Kohler

#4. The polymerase chain reaction is

All of the above

DNA amplification technique

DNA sequencing technique

DNA degradation technique

#5. Which of the following is the basic requirement of PCR reaction?

All of the above

A heat-stable DNA polymerase

DNA segment to be amplified

Two oligonucleotide primers

#6. Which of the following is not a thermostable polymerase?

Taq polymerase

DNA polymerase III

pfu polymerase

Vent polymerase

#7. Which of the following statements is accurate for the PCR – polymerase chain reaction?

Millions to billions of desired DNA copies can be produced from microgram quantities of DNA

Automated PCR machines are called thermal cyclers

All of the above

A thermostable DNA polymerase is required

#8. Restriction endonucleases are utilised in genetic engineering as

Molecular cement for combining DNA bits into long chains

Molecular scalpels for cutting DNA bits into long chains

Molecular degradation to DNA break up

Molecular build up at nucleotides

#9. The enzyme used in PCR technology is

Taq polymerase

Reverse transcriptase

Both b and c

RNA polymerase

#10. Restriction endonucleases are present in mammalian cell for degradation of DNA when the cell dies

Are used for in vitro DNA synthesis

Are used in genetic engineering for ligating two DNA molecules

Are synthesized by bacteria as part of their defence mechanism

None of the above

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Quiz 27-October-2022

#1. Which of the following technique is of no use in protein engineering?

#2. Which of the following is the first and the most important step in the polymerase chain reaction?

#3. The PCR technique was developed by

#4. The polymerase chain reaction is

#5. Which of the following is the basic requirement of PCR reaction?

#6. Which of the following is not a thermostable polymerase?

#7. Which of the following statements is accurate for the PCR – polymerase chain reaction?

#8. Restriction endonucleases are utilised in genetic engineering as

#9. The enzyme used in PCR technology is

#10. Restriction endonucleases are present in mammalian cell for degradation of DNA when the cell dies

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