Hepatitis E virus
One of the common virus responsible for liver diseases in human beings is Hepatitis E Virus (HEV). These days with increase in food and water contamination several chronic and acute liver diseases have also increased.
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Therefore, easiest method of isolation of the most common virus responsible for liver malfunction is of vital importance. In total there are four strains genotype 1, genotype 2, genotype 3, genotype 4. Among them first two that is genotype 1 and genotype 2 are responsible for human infection. They are fatal sometimes especially for pregnant women.
Several cell lines (mostly hepatocytes) have already been developed previously for HEV propagation and biological activity study, but production of HEV in these cell lines is suboptimal.
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Therefore, a method was developed by S. Lin and colleagues recently from clinical HEV fecal specimens and published in Current Protocols in Microbiology in February 2018. They cultured the clinical specimens, isolated and propagated HEV and then quantified HEV RNA by RT-qPCR.
This protocol about HEV can be of great help for those researchers who are involved in various research activities on isolation and production of Hepatitis E virus.
Lin, S., Yang, L., & Zhang, Y.-J. (2018). Hepatitis E virus: Isolation, propagation, and quantification. Current Protocols in Microbiology,48, 15L.1.1–15L.1.15. doi: 10.1002/cpmc.50
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