BACKGROUND
Study of cultural characteristics of microorganism is done with a purpose to distinguish different microorganisms into various taxonomic groups. So the objective of this experiment is to understand different characteristics of microorganisms and to classify them into various groups basing on their characteristics.
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REQUIREMENTS
- Agar plates containing culture
- Agar slants containing culture
- Broth culture
PROCEDURE
Agar plates characterization of microorganism
Observe the culture for colour, opacity, configuration, margin and elevation.
Agar slants characterization
Cultures are examined for
Amount of growth: nil, less, moderate and profusely1
Pigmentation: Pigmentation within the organism and pigment that diffuses into the nearby medium.
Opacity: Opaque, transparent and translucent depending upon the amount of growth.
Different forms: Echinilate, beaded, arborescent, rhizoid, effuse, filiform.
Broth culture characterization
Surface: Membranous, flocculent, thinner
Subsurface: Turbid, flaky, granular, flocculent.
CONCLUSION
The cultural characteristics of microorganisms will helps in differentiating microorganisms on basis of their physical appearances.
REFERENCES
- Dirr MA. 1990. Available at: cabdirect.org.
Also read:
- Effect of Inhibitors on Enzyme Activity
- Biochemical Test (IMViC reactions)
- Study of bacterial morphology by acid-fast staining (Ziehl and Neelsen staining method)
- Mutagenesis with Ethidium bromide/NTG or EMS
- Protein denaturation by egg albumin method (in vitro)
- Estimation of white blood cell count (TLC/ Total Leucocyte Count)
- Analgesic activity study of drugs by tail-flick method
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