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BACKGROUND
Sub culturing is the method of transferring microorganisms from one growth container to another, thereby offering them with fresh supply of nutrients either in a solid or a liquid medium.So the basic objective of this test is to prepare subculture of bacteria and fungus.
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REQUIREMENTS
Materials: Bacterial Culture in Nutrient agar plate
Fungal culture in Potato Dextrose agar plate
Sterile medium
Nutrient agar plate
Potato dextrose agar
Requirements: Inoculating loop
Bunsen burner
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PROCEDURE
Subculturing method of bacteria
Inside the laminar airflow, take the plate cultures containing the isolated colonies of bacteria grown on them. Pick up an isolated colony or more colonies in the inoculating loop. And then close this plate. Now transfer this single colony or few colonies of bacteria into the new plate by streaking in a zig-zag method. To avoid contamination, it is advisable to lift the lid of the plate to minimum. Then close the lid, invert it and incubate the plate for 24-48 hours.1
Subculturing method of fungus
Inside the laminar airflow, take the plate cultures containing fungus. Open it very less as the spores may come out.Then show the tip of the inoculating loop in the Bunsen burner to sterilize it.Then a loopful of fungal culture and streak it in the new sterile PDA plate.Then close the plate and incubate it for 48-72 hours.
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CONCLUSION
By following the above method we can easily subculture the bacteria and fungus for long term preservation and study the various properties of bacteria and fungus.
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REFERENCES
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Miransari M, Smith D. Biotechnology, 2009. Available at: docsdrive.com.
