BACKGROUND
Proteins are biological macromolecules which play diverse functional role starting from structural (collagen, elastin etc.), immune system molecules (antibody), catalyst (enzymes), oxygen transport (haemoglobin), locomotion (actin/myosin of muscle), poison (venom) and many more.1
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Identification of protein molecules is important in various contexts, identification of certain category of antibody in body specifically identifies the type of infection, and similarly presence of proteins in urine identifies the problem associated with abnormal physiology. Here we will discuss the basic method to identify presence of protein in a sample.
REQUIREMENTS
Sample: Egg white, Milk, Blood Plasma etc.
Apparatus: Test tube/Micro centrifuge tube (MCT),
Materials: Biuret reagent2
PROCEDURE
Collect a test tube, add 2 ml of Biuret reagent and mix it well.
CONCLUSION
If the colour of the sample changes to pink-purple, it indicates presence of protein.
REFERENCES
- Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J. Molecular cell biology (Vol. 3). New York: Scientific American Books. 1995.
- Sapan CV, Lundblad RL, Price NC. Colorimetric protein assay techniques. Biotechnology and applied Biochemistry, 1999;29(2):99-108.
Also read:
- Determination of Km and Vmax of Amylase
- Estimation of protein by Folin Lowry method
- Isolation of Auxotrophic Antibiotic Resistant mutant by Induced mutagenesis in Bacteria by Replica plating technique
- Isolation and enumeration of phosphate solubilising bacteria from different habitats
- Isolation of symbiotic N2-fixing bacteria from root nodules from leguminous plants
- Study of local anesthetics by Infiltration anaesthesia in guinea pig
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