BACKGROUND
Carbohydrates are the chief biological fuel in a biological system. In plants, bacteria and fungi carbohydrate molecules and their derivatives can be observed as structural elements. In addition to the above mentioned roles, the carbohydrate molecules have been known to play important role in immune signaling (antigen) in studied animals.1
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The diverse functionality of carbohydrate molecules charm researchers to investigate them further. Apart from the important functional roles of carbohydrate molecules, in certain cases, carbohydrate molecules identified as human pathological biomarkers (such as Diabetes Mellitus).
The afore mentioned biological roles makes the carbohydrate molecules important for study, so methods have been developed to identify the carbohydrate types and also to quantify them.
Basically carbohydrates can be grouped into three major categories i.e. i) monosaccharaides, ies and disaccharides and ii) polysaccharides.
Aim of this experiment is to test carbohydrate contents.
REQUIREMENTS
Sample: Biological sample,
Fruit juice etc
Reagents: Lugol’s Reagent2
Benedict’s reagent
Apparatus: Test tube,
Micro-centrifuge tube (MCT),
Pipette,
Disposable tips
PROCEDURE
Add starch (complex sugar) solution to Lugol’s solution. The mixture colour will be changed from yellowish brown to purple/blue/black.
Add Benedicts solution to the sample solution. Benedicts solution (blue) is used to identify the simple carbohydrates. Heating carbohydrate solution with benedicts solution alters the color (brick red) of the solution.
CONCLUSION
Infer the presence of different carbohydrate molecules in different samples (A, B, C, D etc).
REFERENCES
- Nelson DL, Lehninger AL, Cox MM. Lehninger principles of biochemistry. Macmillan. 2008.
- Smith AM, Zeeman SC. Quantification of starch in plant tissues. Nature Protocols, 2006;1(3):1342-5.
Also read:
- Estimation of glucose in urine by Benedict’s method
- Test for Proteins
- Determination of Km and Vmax of Amylase
- Estimation of protein by Folin Lowry method
- Determination of saponification value of the given oil/fat
- Determination of saponification value of fat (coconut oil)
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