Estimation of total creatinine in blood by colorimetric analysis
Creatinine is a byproduct of muscle energy metabolism that is easily extracted by the kidney. It is produced at a constant rate and depends on the muscle mass of the individual.
NaOH
Determination of pKa value by Half Neutralization/ Henderson Hasselbalch equation
The mixture acts as a buffer solution. During the course of titration acid concentration decreases and salt concentration goes on increasing. During any stage of titration the pH of buffer solution is explained by the Henderson –Hasselbalch equation.
