Isolation of Genomic DNA from E. coli
Isolation of genomic DNA is one of the most important and common experiment that is carried out in molecular biology and includes the transition from cell biology to molecular biology.
Microbiology
Effect of Temperature, pH, Different Concentration of Glucose and Salt on Growth of E. coli
E. coli is a gram negative, rod shaped bacteria and is one of the common inhabitants of gastrointestinal tract of warm-blooded animals.
Study of Polyploidy in Onion Root Tip Bycolchicine Treatment
A normal cell cycle occurs because of cell cycle and the primary function of cell cycle is duplicating the chromosomal DNA, which further divides into two identical daughter cells.
Calculation of Generation Time and Specific Growth Rate of Bacteria from the Graph Plotted with the Given Data
The basic objective of this experiment is to calculate the generation time and specific growth rate of bacteria from the graph plotted with a given set of data.
Cytochemical Staining of DNA
Felugen reaction or cytochemical staining of DNA is a method of identifying DNA or other chromosomal material in a given cell specimen.
Common Biochemical Tests in Microbiology: Action on Litmus Milk
Milk is a very complicated source of nutrition that consists of protein in an aqueous solution of lactose and minerals. When bacteria are inoculated in this milk, the enzymes secreted by bacteria may bring about changes in the media. Litmus is added identify any changes in the pH.
Common Biochemical Tests in Microbiology: Methyl Red Test
Methyl-Red Test is one of the important biochemical test performed on bacterial species for studying about the ability of an organism to perform mixed acid fermentation from given glucose. It is a part of IMViC test.
Common Biochemical Tests in Microbiology: Indole Test
Indole test is one of the important biochemical test that is done on bacterial species for detection of organism’s capability to degrade the amino acid tryptophan and form indole.
To study the isolation of pure cultures by using streak plate method
In microbiology one of the fast-qualitative methods for the isolation of pure culture is streak plate method. Although there is a number of streaking method but the most common method is quadrant streak method.
To determine total count of microorganisms in a culture (liquid) by using direct microscopy method (haemocytometer method)
Hemocytometer is used exclusively for counting the number of cells in a liquid medium. So the objective of this method is to count the number of microorganisms by using hemocytometer.
