Study of Enzymatic Hydrolysis of Starch

2/5 - (2 votes)

[ps2id id=’background’ target=”/]

BACKGROUND

Enzymes are responsible for speeding up reactions and are mostly synthesized in living cells. A study of enzymatic hydrolysis of starch will give knowledge about the specific reactions of enzyme.¹There are several factors like temperature, pH etc that affects the reaction.

So, the basic objective of this test is to study the hydrolysis of starch by a specific enzyme alpha-amylase in saliva.

[ps2id id=’requirements’ target=”/]

REQUIREMENTS

Reagents: Starch solution – 1%

Phosphate buffer solution

Saliva

Iodine solution

Apparatus: Water bath

Watch glass

Test tubes

[ps2id id=’procedure’ target=”/]

PROCEDURE

Take four test tubes and to it add 1 ml of 1% starch solution and 1ml phosphate buffer solution. In a water bath place all test tubes at temperatures 0^oC, 25^oC, 37^oC and 95^oC.² Then leave the test tune for few minutes and then add about 1 ml of diluted saliva in each and every test tube. Now take 4 separate watch glasses and to it add iodine solution 1 drop. In this iodine solution take 1 drop from each test tube and add to the iodine to check if any starch is left or all hydrolyzed.^3,4 If any starch is left then it turns the iodine solution to blue colour. Measure the time required for hydrolysis of starch in all test tubes.

[ps2id id=’conclusion’ target=”/]

CONCLUSION

Hydrolysis of starch is carried out in large number of ways.

[ps2id id=’references’ target=”/][ps2id id=’1′ target=”/]

REFERENCES

Bender H, Wallenfels K. Pullulanase (an amylopectin and glycogen debranching enzyme) from Aerobacter aerogenes. Methods Enzymol. 1966;8:555–5.
Bollag DM, Edelstein SJ (1991) Protein methods. J Wiley, New York.
Carle GF, Olson MV. An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA. 1985;82:3756–60.
Charalambous BM, Keen JN, McPherson MJ. Collagen-like sequences stabilize homotrimers of a bacterial hydrolase. EMBO J. 1988;7:2903–9.