Quiz 27-October-2022

#1. Which of the following is the basic requirement of PCR reaction?

DNA segment to be amplified

A heat-stable DNA polymerase

Two oligonucleotide primers

All of the above

#2. Which of the following is not a thermostable polymerase?

DNA polymerase III

pfu polymerase

Vent polymerase

Taq polymerase

#3. Restriction endonucleases are utilised in genetic engineering as

Molecular scalpels for cutting DNA bits into long chains

Molecular degradation to DNA break up

Molecular build up at nucleotides

Molecular cement for combining DNA bits into long chains

#4. The PCR technique was developed by

Kohler

Altman Kary Mullis

Milstein

Altman

#5. Restriction endonucleases are present in mammalian cell for degradation of DNA when the cell dies

Are synthesized by bacteria as part of their defence mechanism

Are used in genetic engineering for ligating two DNA molecules

Are used for in vitro DNA synthesis

None of the above

#6. Which of the following is the first and the most important step in the polymerase chain reaction?

None of the above

Denaturation

Annealing

Primer extension

#7. Which of the following technique is of no use in protein engineering?

Gene cloning

Lyophilization

Protein sequencing

X-ray crystallography

#8. Which of the following statements is accurate for the PCR – polymerase chain reaction?

A thermostable DNA polymerase is required

All of the above

Automated PCR machines are called thermal cyclers

Millions to billions of desired DNA copies can be produced from microgram quantities of DNA

#9. The polymerase chain reaction is

DNA sequencing technique

All of the above

DNA amplification technique

DNA degradation technique

#10. The enzyme used in PCR technology is

Both b and c

RNA polymerase

Reverse transcriptase

Taq polymerase

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Quiz 27-October-2022

#1. Which of the following is the basic requirement of PCR reaction?

#2. Which of the following is not a thermostable polymerase?

#3. Restriction endonucleases are utilised in genetic engineering as

#4. The PCR technique was developed by

#5. Restriction endonucleases are present in mammalian cell for degradation of DNA when the cell dies

#6. Which of the following is the first and the most important step in the polymerase chain reaction?

#7. Which of the following technique is of no use in protein engineering?

#8. Which of the following statements is accurate for the PCR – polymerase chain reaction?

#9. The polymerase chain reaction is

#10. The enzyme used in PCR technology is

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