Quiz 02-November-2022

#1. At what temperature does annealing of DNA and primer take place?

96°C

54°C

74°C

42°C

#2. Which of the following statement is incorrect regarding HAT medium

Aminiopterin in the HAT medium blocks de novo pathway of nucleotide synthesis

HAT medium is a selective medium

Hypoxanthin is converted to guanine by HGPRT enzyme

Salvage pathway requires aminopterin and thymidine

#3. The hybridomas are made by

Fusing T cells with myeloma cells

Fusing T helper cells with myeloma cells

Fusing B memory cells with myeloma cells

Fusing B cells with myeloma cells

#4. The technology used for the production of monoclonal antibodies is

Hybridoma technology

Suspension culture

Mass culture technology

Monoclonal and Polyclonal Antibodies

#5. Hybridoma technology was developed by

Khorana and Nirenberg

Khorana and Korenberg

Kohler and Milstein

Beedle and Tautum

#6. Natural humoral immune response against a pathogen leads to the production of

Monoclonal antibodies

None of these

Macrophages

Polyclonal antibodies

#7. Primers used for the process of polymerase chain reaction are

Single-stranded DNA oligonucleotide

Double-stranded DNA oligonucleotide

Single-stranded RNA oligonucleotide

Double-stranded RNA oligonucleotide

#8. How many DNA duplexes can be obtained from one DNA duplex after 4 cycles of PCR?

#9. The polymer chain reaction is used for

Constructing RAPD maps

Amplifying gene of interest

All of the above

Detecting the presence of the transgene in an organism

#10. Which of the following is an application of polymer chain reaction?

Site-directed mutagenesis

All of the above

Site-specific recombination

Site-specific translocation

Finish

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