Microbiological assay of antibiotics pdf, antibiotic assay method, cylinder-plate method procedure, turbidimetric method of antibiotic assay, cylinder-plate or cup plate method, tube assay method, cylinder-plate method for microbiological assay
BACKGROUND
The microbiological assay of antibiotics is done by comparing the zone of inhibition formed by the microorganisms to a specific concentration of antibiotics having a known activity. There are different types of methods for microbiological assay of antibiotics like cup plate method and disc diffusion method.
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Microbiological assay of antibiotics
In the cup plate method, antibiotic containing cylinder is diffused into the agar layer containing the microorganisms. The zone is formed around the cylinder. The other method is disc diffusion method where zone of inhibition is measured around the antibiotic disc. The basic objective is to study various methods of microbiological assay.1
REQUIREMENTS
Organism: Test microorganism
Chemicals: DMF solvent
Antibiotics
Nutrient Agar medium
Antibiotic disc
Apparatus: Borer
Petri plates
PROCEDURE
Cup-plate method1
Prepare nutrient agar plate inoculated with test organism, with a depth of 4-5mm and then allow it to solidify. Divide the NA plate into four equal portions. Then with the help of a sterile borer make four cavities one in each portion. Then fill three cavities with antibiotic solution and in one fill the standard solution. Slowly incubate the plates at 370C for 24 hours. After incubation measure the zone of inhibition.
Disc diffusion method2
Prepare nutrient agar plates and with the help of a cotton swab spread the broth solution containing the test microorganism uniformly. Then with the help of a sterile forceps put the required antibiotic disc slowly above the plate. Then incubate the plate at 370C for 24 hours. Measure the one of inhibition formed.
CONCLUSION
By measuring the zone of inhibition, the antimicrobial activity of the microorganism.
REFERENCES
- Ruth E. Miller, S. Brandt Rose American Journal of Clinical Pathology. 2015;11(1):414-24.
- Drew WL, Barry AL, Toole RO, Sherris JC. Applied Microbiology, Am Soc Microbial. 1972.
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FAQs
In the cup plate method, antibiotic containing cylinder is diffused into the agar layer containing the microorganisms. The zone is formed around the cylinder.
The microbiological assay of antibiotics is done by comparing the zone of inhibition formed by the microorganisms to a specific concentration of antibiotics having a known activity. There are different types of methods for microbiological assay of antibiotics like cup plate method and disc diffusion method.
