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BACKGROUND
Gastric juice is secreted by the glands located in the lining of the stomach. Total acidity of gastric juice is the expression of gastric acidity. The acid part of the gastric juice is due to HCl which may be present either freely or in combination with other proteins. Sometimes lactic acids, carbon dioxide and fatty acids also contributes to the gastric acidity. All these contribute to the total acidity of gastric juice.
So the objective of this experiment is to estimate the total acidity of gastric juice.
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REQUIREMENTS
Reagents: 0.1N NaOH
Topfer’s reagent: 0.5 g dimethyl aminobenzene + 100 ml of 95% ethanol
1% alcoholic solution of phenolphthalein.
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PROCEDURE
In a clean vial take 0.1 ml of gastric juice and to it add about 0.9 ml of distilled water. Then perform titration of this prepared mixture by using Topfer’s reagent as an active indicator until the colour changes to salmon pink. Now to this add few drops of phenolphthalein and titration is continued till the colour changes to pink colour.1 The reading of the burette in this point gives the total acidity. The final amount of HCl is calculated as gastric juice (pEq/4 hrs of gastric juice).2
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CONCLUSION
Estimation of total acidity of gastric juice gives idea about whether or not the body contains right amount of acid. If more acid is present then measures are taken to neutralize it.
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REFERENCES
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Baird, M. M., Oampbell, J.M., and Hern, J.R., The importance of estimating chlorides in the fractional test meal samples, Guy1s Hosp. Reports, 74:23, 1924.
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Baird, M. M., Oampbell, J. M., and Hern, J. R., Gastric secretion, physique and physical fitness, Guyts Hesp. Reports, 74:339, 1924.
