BACKGROUND
Carbohydrate molecules plays diverse role in biological system.1 In certain cases, carbohydrate molecules identified as human pathological biomarkers (such as Diabetes Mellitus). The estimation of the glucose in urine gives information on the patient DM control. Here we will use Benedict’s method to determine the concentration of urine sugar in patient urine sample.1
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REQUIREMENTS
Sample: Urine
Reagents: Benedict’s reagent2
Apparatus: Test tube,
Micro-centrifuge tube (MCT),
Pipette,
Disposable tips,
Spectrophotometer
PROCEDURE
Add Benedict’s solution to the urine sample solution. Benedicts solution (blue) is used to identify the simple carbohydrates. Heating carbohydrate solution with benedicts solution alters the color (brick red) of the solution. Record the OD 575 in spectrophotometer. Prepare a standard curve using known concentration of sugar in solution.
CONCLUSION
Fit the sample data in the OD575 obtained from the urine sample on the standard curve to find the concentration of the sugar in urine by Benedict’s method.
REFERENCES
- Nelson DL, Lehninger AL, Cox MM. Lehninger principles of biochemistry. Macmillan, 2008.
- Benedict SR. The detection and estimation of glucose in urine. Journal of the American Medical Association, 1911;57(15):1193-4.
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