Effect of saline purgative on frog intestine

BACKGROUND

Saline Purgative

Saline purgatives are the salts comprising of highly charged ions and do not crosses cell membrane freely. They remain inside the lumen and retain water through osmotic forces. They increase the volume of the contents of the bowel, stretch the colon and produces normal stimulus for contraction of the muscle that leads to defecation. The aim of the present study is to examine the effect of saline purgative on frog intestine.¹

REQUIREMENTS

Animal: Frog

Reagents: 0.9% to 0.45% of saline (hypotonic), 27% Magnesium sulphate (hypertonic), Frogs Ringer solution (isotonic)

Instruments used: Frog’s board, pithing needle, dissecting instruments, needle with thread, tuberculin syringe with needle.

PROCEDURE

Pith the frog and place it on a dissecting board. Expose the abdominal cavity and carefully trace the small intestine. Make the small intestine into three compartments by tying threads of different colours in such a way that no fluid can move from one compartment to the other. Inject 0.2 ml of each hypotonic solution into first compartment, 0.2 ml of hypertonic solution to second compartment and 0.2 ml of isotonic solution into third compartment. Wait for 20 minutes and the observations are to be recorded.

OBSERVATION

Drug	Compartment	Effect
Hypotonic solution (0.2 ml of 0.9% of saline)	First compartment	Shrunken
Hypertonic solution (0.2 ml of 27% magnesium sulphate)	Second compartment	Swollen
Isotonic solution (0.2 ml of frogs Ringer solution)	Third compartment	No change

CONCLUSION

Hypotonic solution causes the fluid to move from lumen into circulation by process osmosis thereby shrinks the tissue. Hypertonic solution moves the fluid from cells into the lumen and swells the tissue and isotonic solution did not shows any fluid movement across the intestinal membrane.

REFERENCES

1. Laxative. Available at: https://www.britannica.com/science/laxative#ref800128. Accessed on 10 November 2016.

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