Determination of Clotting Time

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BACKGROUND

Clotting Time

Clotting time is defined as the time interval in between onset of bleeding and appearance of semisolid mass i.e. clot. Normal value of clotting time is 3-4 minutes. This is determined using two methods viz. Capillary glass method and Wrights Co-agulometer.

Whenever a great blood vessel ruptures bleeding continues. In a few minutes blood looses its fluidity and sets into a semisolid mass. The mass is referred as clot and the phenomenon as coagulation.¹

Aim:

The aim of the experiment is to determine the clotting time of the subject.

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REQUIREMENTS

Spirit,
Cotton,
Needle,
Capillary tube,
Stop watch.

PROCEDURE

Capillary glass method

The finger tip of the subject is sterilized and a bold prick Is made in the finger tip with a sterilized needle to have free flow of blood.

The blood coming out of the puncture is sucked into a capillary glass tube of 15 cm long. Then the tube is kept undisturbed horizontally for about 1-2 minutes. A small bit of the glass tube is broken off every 30 seconds until a fine thread of clotted blood appears while the capillary tube is broken.

When the thread appears the stop watch is stoped. This gave us the clotting time. The period in between appearance of blood in finger and formation of clot was taken as clotting time.

CONCLUSION

The clotting time of the subject was found to be__________minutes.

REFERENCES

Kale SR, et al. Haematology, Practical Human Anatomy And Physiology. Nirali Prakashan, Eight Edition, 2002: 32-33.

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