[ps2id id=’background’ target=”/]
BACKGROUND
There are three types of cells in the blood stream namely leucocytes (white blood cells), erythrocytes (red blood cells) and thrombocytes (platelets). The complete blood count is the most common blood investigations advised by the doctor to know the overall health of the subject.1
The hematology analyzers are used to detect the total count of blood cells and also to diagnose diseases of the blood cells and to monitoring the health condition of the patient. Some of the complex analyzers also measure the cell morphology and helps in diagnosis of rare blood disorders. The technologies used in hematology analyzers are electrical impedance, flow cytometry and fluroscent flow cytometry.2
The aim of the experiment is demonstration of total blood count by cell analyzer.
[ps2id id=’requirements’ target=”/]
REQUIREMENTS
Subjects: Human
Apparatus: Hematology analyzer
Blood sample
[ps2id id=’procedure’ target=”/]
PROCEDURE
The subject is either in sitting or lying down position. The suitable vein is punctured by placing a tourniquet 3- 4 inches above the puncture site. The blood is collected and sent for analysis.
The following technologies are used in hematology analyzers.
Electrical impedance
This is known as Coulter principle, used in most of the analyzers. The blood is passed between two electrodes through a narrow aperture which can pass a single cell at a time. The change in impedance is proportional to the cell volume, which in turn measures the cell count and volume.2
Flow cytometry
It is used to determine 5-part WBC differentials. The single stream of light passing through the laser beam measures cell granularity, diameter and inner complexity. The addition of fluroscent reagents to flow cytometry renders it fluroscent flow cytometry which measures specific cell populations namely platelets, nucleated RBCs and reticulocytes.2
[ps2id id=’conclusion’ target=”/]
CONCLUSION
Complete blood count analysis is used in diagnosing diseases, monitor health conditions and know the prognosis of medical treatments.
[ps2id id=’references’ target=”/][ps2id id=’1′ target=”/]
REFERENCES
-
En.m.wikepedia.org. Accessed on 29 March 2018
-
https://www.labcompare.com/10-Featured-Articles/162042-Hematology-Analyzers-From-Complete-Blood -Counts-to-Cell-Morphology. Accessed on 29 March 2018.
