Microbiology

Cytochemical Staining of DNA

Felugen reaction or cytochemical staining of DNA is a method of identifying DNA or other chromosomal material in a given cell specimen.

Isolation of Mutants of Bacteria by UV Exposure

When there is a hereditary change in the base sequence of a DNA it is called mutation. Very few mutations are either neutral or beneficial to organisms but usually they cause harm to living beings as that result

Cultivation of Edible Mushrooms

There is no doubt that mushrooms are super delicious but some of the gourmet varieties are very expensive and is quite difficult to get it fresh.

Plaque Assay for Coliphage

Bacteriophages are obligate intercellular parasites. They utilize the biosynthetic machinery of the host for their multiplication. They make an entry into the bacterial cell by first making a landing on the bacterial cell wall

Isolation of Rhizobium from Root Nodule

Rhizobium lives in soil and in the roots of legumes and non-leguminous plants. Bacteria exclusively depends on the root exudates that facilitates the growth of the rhizobial cells.

Methylene Blue Reduction Test for Milk

One of the best medium for growth of microorganism is milk. Both raw milk as well as the pasteurized milk contain varied microorganisms. Thus, one of the popular tools for determination of the milk quality is development of Methylene Blue Reductase.

Biochemical Tests for Enteric Organisms

Prepare urea agar slant and streak in its surface with isolated colonies of the Enteric organism. Incubate the slant in a incubator for about 48 hours at a temperature of 35C to 37C.