Cell viability test by Trypan Blue
Trypan Blue dye is used to differentiate viable cells present in a cell suspension from dead cells based on the concept that viable cells have intact cell membranes that exclude
Animal Cell Culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.
Differentiation of live cells from dead cells by Giemsa stain
A thiazine-eosine buffered solution, Giemsa stain is designed to deliver coloration for type of cells. Giemsa’s solution is a mixture of methylene blue, eosin, and Azure B and available in a powder form.
Preparation of single cell suspension from spleen and thymus
Spleen and thymus are the lymphoid organs of an animal starting from lower vertebrate like fish to higher vertebrate like human. Therefore, culture of cells derived from these lymphoid organs will help to understand
Subculture of cells from primary culture
Methods of extracting cells from an animal and subsequent growth in an artificially controlled environment termed as primary cell culture. Subculture is a new culture originated from the primary cell culture.
MTT assay for cell viability and growth
The growth of cells can be determined by the reduction of tetrazolium salts in MTT assay. Metabolically active cells reduce the yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2,
Primary cell culture from chick embryo
Variety of cells isolated and culture directly from tissues is defined as primary culture, it contains progenitor and differentiated cells. Cells in the primary culture has a limited life span so more cells
