In a catalogue, there are a large number of products, like chemical compounds, reagents that need proper navigation before ordering. In a hurry, we often end up ordering for wrong products like a reverse complement primer or an enantiomer of a receptor ligand. This all results in both loss of time and money.
It is another major mistake that can take a lot of time. When we draw our conclusions depending on the improper analysis of results this leads to misinterpretation of our data. Therefore, the best solution is to decide the process of analysis from beginning only.
When the spinning of the solubilized sample is not done completely before immunoprecipitation then it results in the non-solubilized membrane components and protein to get aggregate and remains in the sample. This will pull down experiments and results in wrong precipitation.
