Arneth Index

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BACKGROUND

The Arneth index describes the nucleus of neutrophils. This helps in detection of diseases. There can be right or left shift of Arneth Index. The neurtophils have lobed nucleus of either two or three. The older neutrophils have more lobes than the younger neutrophils.

The left shift (larger percentage of neutrophils with fewer lobes) indicates presence of infection, malignant tumours, hemolytic crisis, myocardial infarction, acidosis etc. The right shift (larger percentage of neutrophils with more lobes) indicates the presence of Vitamin B12 or folate deficiency, chronic uremia, liver diseases etc.1

The aim of the experiment is to determine Arneth Index.

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REQUIREMENTS

Subjects:     Human

Apparatus:  Blood sample

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PROCEDURE

Collect the blood sample from the subject and prepare a blood smear. Stain the smear and examine the stained smear under microscope.

The grouping of neutrophils is done based on the number of lobes present in the nuclei.

Group 1:     Horseshoe shape nuclei

Group 2:    Bilobed nuclei

Group 3:     Trilobed nuclei

Group 4:    Four lobed nuclei

Group 5:     Five or more lobed nuclei2

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CONCLUSION

The Arneth index /count help in diagnosis of various diseases of body.

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REFERENCES

  1. https://en.m.wikipedia.org/wiki/Arneth_count. Accessed on 16 June 2018
  2. https://www.ncbi.nih.gov.pmc/articles/PMC382513/PDF/canmedj00106-0078.pdf. Accessed on 16 June 2018.