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BACKGROUND
The Arneth index describes the nucleus of neutrophils. This helps in detection of diseases. There can be right or left shift of Arneth Index. The neurtophils have lobed nucleus of either two or three. The older neutrophils have more lobes than the younger neutrophils.
The left shift (larger percentage of neutrophils with fewer lobes) indicates presence of infection, malignant tumours, hemolytic crisis, myocardial infarction, acidosis etc. The right shift (larger percentage of neutrophils with more lobes) indicates the presence of Vitamin B12 or folate deficiency, chronic uremia, liver diseases etc.1
The aim of the experiment is to determine Arneth Index.
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REQUIREMENTS
Subjects: Human
Apparatus: Blood sample
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PROCEDURE
Collect the blood sample from the subject and prepare a blood smear. Stain the smear and examine the stained smear under microscope.
The grouping of neutrophils is done based on the number of lobes present in the nuclei.
Group 1: Horseshoe shape nuclei
Group 2: Bilobed nuclei
Group 3: Trilobed nuclei
Group 4: Four lobed nuclei
Group 5: Five or more lobed nuclei2
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CONCLUSION
The Arneth index /count help in diagnosis of various diseases of body.
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REFERENCES
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https://en.m.wikipedia.org/wiki/Arneth_count. Accessed on 16 June 2018
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https://www.ncbi.nih.gov.pmc/articles/PMC382513/PDF/canmedj00106-0078.pdf. Accessed on 16 June 2018.