Antibiotic Susceptibility Test

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BACKGROUND

Antibiotic susceptibility testing is one of the monotonous processes in all microbiology laboratory. Antibiotics are the substances produced by microorganisms that can kill or inhibit the growth of another organism. Therefore, with increase in requirement of large number of antimicrobials for treatment of various infections the importance of antibiotic susceptibility test increased.1

The objective of these test is to know the susceptibility of a specific microbial species against diverse antibiotic agents.

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REQUIREMENTS

Apparatus:            Petri plate

Inoculation loop

Chemicals:       Saline solution

MHA (Muller-Hinton Agar) plate or NA (Nutrient Agar) plate

Antibiotic discs

Miscellaneous:     Cotton swab

Ruler

Tooth pick

Incubator

Instruments:        Bunsen burner

Laminar air flow

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PROCEDURE

Select one pure culture of the organism to be tested.

Mix a colony of these cultures in the sterile solution and mix it carefully so that no content of the colony is visible.

Now take a cotton swab and dip it in the sterile solution containing organism.

Then take a MHA plate or NA plate, and spread the culture over it from the cotton swab.

Spread it evenly all over the plate. Next place the antibiotic disc over the surface of the plate with the help of sterile forceps. Slightly press the disc into the agar surface using an inoculation loop.

Now slowly invert the plate and keep it in incubator at 37oC for 24 hours.2

Lastly, with the help of ruler measure the zone of inhibition and compare it with the standard table to know whether the particular organism is sensitive or resistant to the antibiotics tested.

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CONCLUSION

The size of zone will give us a perfect idea about how the microorganism reacts to the antibiotic. Accordingly, the antibiotic can be used for inhibition of infections caused by that organism.

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REFERENCES

  1. Jorgensen JH, Turnidge JD. Manual of clinical Microbiology , 2015.

  2. Reller LB, Weinstein M, Jorgensen JH. Clinical Infectious, 2009.

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